Bacteria:Article Title: Antimicrobial compounds
Article Snippet: 172-176° C.; νmax/cm−1 3272 (NH), 1708 (C═O), 1637 (C═O), 1530 (NH bend), 1226 (P═O), 1165 (P—O), 1020 (P—O), 966 (P—O); 1H NMR (300 MHz, CDCl3) δH 1.16-1.36 (12H, [m, CH3-2], [m, CH3-6], [m, 2×OCH2CH3]), 1.36 (9H, s, C(CH3)3), 1.82-2.01 (2H, m, CH2-10), 2.04 (3H, s, CH3-12), 2.49 (2H, t, J=9.0 Hz, CH2-11), 4.00-4.12 (4H, m, 2×OCH2CH3), 4.21 (1H, m, CH-9), 4.33-4.43 (1H, m, CH-1), 4.45-4.53 (1H, m, CH-5), 5.40 (1H, d, J=9.0 Hz, NH-13-A), 5.44 (1H, d, J=6.0 Hz, NH-13-B), 6.85 (1H, d, J=6.0 Hz, NH-7-A), 6.92 (1H, d, J=6.0 Hz, NH-7-B), 7.07 (1H, d, J=9.0 Hz, NH-3-A), 7.16 (1H, d, J=9.0 Hz, NH-3-B); 13C NMR (75 MHz, CDCl3) δC 14.3 (CH3-2-A), 14.3 (CH3-2-B), 15.4-15.5 (2×OCH2CH3), 17.7 (CH3-6), 27.3 (C(CH3)3), 29.2 (CH2-11-A), 29.3 (CH2-11-B), 30.8 (CH2-10-A), 30.8 (CH2-10-B), 39.9 (d, JP—C=156.8 Hz, CH-1-A), 40.0 (d, JP—C=156.8 Hz, CH-1-B), 47.9 (CH-5-A), 48.0 (CH-5-B), 52.6 (CH-9), 61.5 (d, JP—C=4.5 Hz, OCH2CH3-A), 61.6 (d, JP—C=4.5 Hz, OCH2CH3—B), 61.7 (d, JP—C=6.8 Hz, OCH2CH3-A), 61.9 (d, JP—C=6.8 Hz, OCH2CH3—B), 79.1 (C(CH3)3), 154.6 (C═O-14), 170.3 (C═O-4 or C═O-8-A), 170.4 (C═O-4 or C═O-8-B), 170.5 (C═O-4 or C═O-8-A), 170.6 (C═O-4 or C═O-8-B). .. The results obtained are presented in Table 5 below TABLE 5 Minimum inhibitory concentrations of antimicrobial compounds I and J against various bacteria species Minimum inhibitory Minimum inhibitory Strain concentration (MIC) of concentration (MIC) of Species reference compound I (μg/mL) compound J (μg/mL) Acinetobacter baumannii ATCC 19606 >8 >8 Burkholderia cepacia ATCC 25416 >8 >8 Enterobacter cloacae NCTC 11936 >8 >8 Escherichia coli NCTC 10418 1 2 Escherichia coli NCTC 12241 1 2 Klebsiella pneumoniae NCTC 9528 0.25 0.5 Providencia rettgeri NCTC 7475 >8 >8 Pseudomonas aeruginosa NCTC 10662 >8 >8 Salmonella Typhimurium NCTC 74 >8 >8 Salmonella Enteritidis NCTC 6676 >8 >8 Serratia marcescens NCTC 10211 0.25 0.5 Yersinia enterocolitica NCTC 11176 0.125 0.5 Enterococcus faecalis NCTC 775 0.063 0.125 Enterococcus faecium NCTC 7171 1 4 Listeria monocytogenes NCTC 11994 >8 >8 Staphylococci epidermidis NCTC 11047 1 2 Staphylococcus aureus NCTC 6571 1 1 Staphylococcus aureus NCTC 11939 >8 >8 (SARM) According to the data presented in table 5 above, it clearly appears that compounds I and J are strongly inhibiting against the tested strains of certain Gram-negative bacteria species and certain Gram-positive bacteria species, notably concerning Escherichia coli, Klebsiella pneumoniae, Serratia marcescens, Yersinia enterocolitica, Enterococcus faecalis, and less inhibiting against other Gram-negative and Gram-positive bacteria species, notably with regard to Acinetobacter baumannii, Burkholderia cepacia, Pseudomonas aeruginosa, Salmonella typhimurium, Salmonella enteritidis, Listeria monocytogenes. .. The results obtained are presented in Table 5 below TABLE 5 Minimum inhibitory concentrations of antimicrobial compounds I and J against various bacteria species Minimum inhibitory Minimum inhibitory Strain concentration (MIC) of concentration (MIC) of Species reference compound I (μg/mL) compound J (μg/mL) Acinetobacter baumannii ATCC 19606 >8 >8 Burkholderia cepacia ATCC 25416 >8 >8 Enterobacter cloacae NCTC 11936 >8 >8 Escherichia coli NCTC 10418 1 2 Escherichia coli NCTC 12241 1 2 Klebsiella pneumoniae NCTC 9528 0.25 0.5 Providencia rettgeri NCTC 7475 >8 >8 Pseudomonas aeruginosa NCTC 10662 >8 >8 Salmonella Typhimurium NCTC 74 >8 >8 Salmonella Enteritidis NCTC 6676 >8 >8 Serratia marcescens NCTC 10211 0.25 0.5 Yersinia enterocolitica NCTC 11176 0.125 0.5 Enterococcus faecalis NCTC 775 0.063 0.125 Enterococcus faecium NCTC 7171 1 4 Listeria monocytogenes NCTC 11994 >8 >8 Staphylococci epidermidis NCTC 11047 1 2 Staphylococcus aureus NCTC 6571 1 1 Staphylococcus aureus NCTC 11939 >8 >8 (SARM) According to the data presented in table 5 above, it clearly appears that compounds I and J are strongly inhibiting against the tested strains of certain Gram-negative bacteria species and certain Gram-positive bacteria species, notably concerning Escherichia coli, Klebsiella pneumoniae, Serratia marcescens, Yersinia enterocolitica, Enterococcus faecalis, and less inhibiting against other Gram-negative and Gram-positive bacteria species, notably with regard to Acinetobacter baumannii, Burkholderia cepacia, Pseudomonas aeruginosa, Salmonella typhimurium, Salmonella enteritidis, Listeria monocytogenes.
Concentration Assay:Article Title: Antimicrobial compounds
Article Snippet: 172-176° C.; νmax/cm−1 3272 (NH), 1708 (C═O), 1637 (C═O), 1530 (NH bend), 1226 (P═O), 1165 (P—O), 1020 (P—O), 966 (P—O); 1H NMR (300 MHz, CDCl3) δH 1.16-1.36 (12H, [m, CH3-2], [m, CH3-6], [m, 2×OCH2CH3]), 1.36 (9H, s, C(CH3)3), 1.82-2.01 (2H, m, CH2-10), 2.04 (3H, s, CH3-12), 2.49 (2H, t, J=9.0 Hz, CH2-11), 4.00-4.12 (4H, m, 2×OCH2CH3), 4.21 (1H, m, CH-9), 4.33-4.43 (1H, m, CH-1), 4.45-4.53 (1H, m, CH-5), 5.40 (1H, d, J=9.0 Hz, NH-13-A), 5.44 (1H, d, J=6.0 Hz, NH-13-B), 6.85 (1H, d, J=6.0 Hz, NH-7-A), 6.92 (1H, d, J=6.0 Hz, NH-7-B), 7.07 (1H, d, J=9.0 Hz, NH-3-A), 7.16 (1H, d, J=9.0 Hz, NH-3-B); 13C NMR (75 MHz, CDCl3) δC 14.3 (CH3-2-A), 14.3 (CH3-2-B), 15.4-15.5 (2×OCH2CH3), 17.7 (CH3-6), 27.3 (C(CH3)3), 29.2 (CH2-11-A), 29.3 (CH2-11-B), 30.8 (CH2-10-A), 30.8 (CH2-10-B), 39.9 (d, JP—C=156.8 Hz, CH-1-A), 40.0 (d, JP—C=156.8 Hz, CH-1-B), 47.9 (CH-5-A), 48.0 (CH-5-B), 52.6 (CH-9), 61.5 (d, JP—C=4.5 Hz, OCH2CH3-A), 61.6 (d, JP—C=4.5 Hz, OCH2CH3—B), 61.7 (d, JP—C=6.8 Hz, OCH2CH3-A), 61.9 (d, JP—C=6.8 Hz, OCH2CH3—B), 79.1 (C(CH3)3), 154.6 (C═O-14), 170.3 (C═O-4 or C═O-8-A), 170.4 (C═O-4 or C═O-8-B), 170.5 (C═O-4 or C═O-8-A), 170.6 (C═O-4 or C═O-8-B). .. The results obtained are presented in Table 5 below TABLE 5 Minimum inhibitory concentrations of antimicrobial compounds I and J against various bacteria species Minimum inhibitory Minimum inhibitory Strain concentration (MIC) of concentration (MIC) of Species reference compound I (μg/mL) compound J (μg/mL) Acinetobacter baumannii ATCC 19606 >8 >8 Burkholderia cepacia ATCC 25416 >8 >8 Enterobacter cloacae NCTC 11936 >8 >8 Escherichia coli NCTC 10418 1 2 Escherichia coli NCTC 12241 1 2 Klebsiella pneumoniae NCTC 9528 0.25 0.5 Providencia rettgeri NCTC 7475 >8 >8 Pseudomonas aeruginosa NCTC 10662 >8 >8 Salmonella Typhimurium NCTC 74 >8 >8 Salmonella Enteritidis NCTC 6676 >8 >8 Serratia marcescens NCTC 10211 0.25 0.5 Yersinia enterocolitica NCTC 11176 0.125 0.5 Enterococcus faecalis NCTC 775 0.063 0.125 Enterococcus faecium NCTC 7171 1 4 Listeria monocytogenes NCTC 11994 >8 >8 Staphylococci epidermidis NCTC 11047 1 2 Staphylococcus aureus NCTC 6571 1 1 Staphylococcus aureus NCTC 11939 >8 >8 (SARM) According to the data presented in table 5 above, it clearly appears that compounds I and J are strongly inhibiting against the tested strains of certain Gram-negative bacteria species and certain Gram-positive bacteria species, notably concerning Escherichia coli, Klebsiella pneumoniae, Serratia marcescens, Yersinia enterocolitica, Enterococcus faecalis, and less inhibiting against other Gram-negative and Gram-positive bacteria species, notably with regard to Acinetobacter baumannii, Burkholderia cepacia, Pseudomonas aeruginosa, Salmonella typhimurium, Salmonella enteritidis, Listeria monocytogenes. .. The results obtained are presented in Table 5 below TABLE 5 Minimum inhibitory concentrations of antimicrobial compounds I and J against various bacteria species Minimum inhibitory Minimum inhibitory Strain concentration (MIC) of concentration (MIC) of Species reference compound I (μg/mL) compound J (μg/mL) Acinetobacter baumannii ATCC 19606 >8 >8 Burkholderia cepacia ATCC 25416 >8 >8 Enterobacter cloacae NCTC 11936 >8 >8 Escherichia coli NCTC 10418 1 2 Escherichia coli NCTC 12241 1 2 Klebsiella pneumoniae NCTC 9528 0.25 0.5 Providencia rettgeri NCTC 7475 >8 >8 Pseudomonas aeruginosa NCTC 10662 >8 >8 Salmonella Typhimurium NCTC 74 >8 >8 Salmonella Enteritidis NCTC 6676 >8 >8 Serratia marcescens NCTC 10211 0.25 0.5 Yersinia enterocolitica NCTC 11176 0.125 0.5 Enterococcus faecalis NCTC 775 0.063 0.125 Enterococcus faecium NCTC 7171 1 4 Listeria monocytogenes NCTC 11994 >8 >8 Staphylococci epidermidis NCTC 11047 1 2 Staphylococcus aureus NCTC 6571 1 1 Staphylococcus aureus NCTC 11939 >8 >8 (SARM) According to the data presented in table 5 above, it clearly appears that compounds I and J are strongly inhibiting against the tested strains of certain Gram-negative bacteria species and certain Gram-positive bacteria species, notably concerning Escherichia coli, Klebsiella pneumoniae, Serratia marcescens, Yersinia enterocolitica, Enterococcus faecalis, and less inhibiting against other Gram-negative and Gram-positive bacteria species, notably with regard to Acinetobacter baumannii, Burkholderia cepacia, Pseudomonas aeruginosa, Salmonella typhimurium, Salmonella enteritidis, Listeria monocytogenes.
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